Evidence of Tautomerism in 2-Aminopurine from Fluorescence Lifetime Measurements
نویسندگان
چکیده
منابع مشابه
Long-wavelength fluorescence from 2-aminopurine-nucleobase dimers in DNA.
When 2-aminopurine (2AP) is substituted for adenine in DNA, it is widely accepted that its fluorescence spectrum is essentially unchanged from that of the free fluorophore. We show that 2AP in DNA exhibits long-wavelength emission and excitation bands, in addition to the familiar short-wavelength spectra, as a result of formation of a ground-state heterodimer with an adjacent, pi-stacked, natur...
متن کاملUltrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes
Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the ...
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A. H. BUIST,* M. MÜLLER,* E. J. GIJSBERS,* G. J. BRAKENHOFF,* T. S. SOSNOWSKI,† T. B. NORRIS† & J. SQUIER‡ *BioCentrum Amsterdam, University of Amsterdam, Department of Molecular Cytology, Kruislaan 316, 1098 SM Amsterdam, The Netherlands †Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Blvd, IST Building, Ann Arbor, MI 48109–2099, U.S.A. ‡Department of Electrical a...
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The pH of cheese affects the texture of curd directly by influencing the hydratation of the caseins; all else being equal, high pH cheeses are softer than more acid cheeses. pH also affects texture and flavour indirectly by affecting the activity of enzymes important to ripening and, in the case of the coagulant, the retention of enzyme in the curd during manufacture [1]. Since low pH is known ...
متن کاملFluorescence properties of 2-aminopurine in human telomeric DNA.
The substitution of 2-aminopurine (Ap) for A7 in the human telomeric sequence d[AGGG(TTAGGG)(3)] resulted in a significant increase in the fluorescence intensity of Ap for the conformational change from duplex to quadruplex.
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ژورنال
عنوان ژورنال: The Journal of Physical Chemistry B
سال: 2004
ISSN: 1520-6106,1520-5207
DOI: 10.1021/jp0490857